Influence of mannoprotein-based reactivation media on the post-lyophilization viability of Rhodotorula mucilaginosa CNMN-YS-10

Abstract

Preserving the long-term viability of microorganisms is a fundamental objective of the National Collection of Nonpathogenic Microorganisms (NCNM). The aim of the study was to evaluate the influence of mannoprotein preparations from Lager beer yeast sediments (MPB) and Merlot red wine (MPV) at concentrations of 1%, 5% and 10% on the cell viability of the Rhodotorula mucilaginosa CNMN-YS-10 strain after long-term preservation by lyophilization. Reactivation with MPB at concentrations of 1% and 10% reduced cell viability to 78.14 ± 2.8%, whereas at the intermediate concentration, viability remained comparable to the control range. Reactivating the culture with MPV, even at the minimum concentration, reduced viability to 78.44%, whereas at higher concentrations, viability remained comparable to the control.