Research on the influence of dilution media on ram sperm cryopreservation

Abstract

In the study, ram sperm from the Moldovan Karakul breed bred in the experimental section of the Scientific Institute of Biotechnology Practices in Animal Husbandry and Veterinary Medicine. Ejaculates with a mobility of no less than 70% and a concentration of 2.0 billion/ml were admitted for processing. For dilution, GTJ, STJ and STJ+ dilution media were used, in which the biologically active preparation extracted from cyanobacteria at the Institute of Microbiology and Biotechnology of the Technical University of Moldova was introduced as an additional component. After resuscitation, the mobility, rectilinear movements of the spermatozoa, acrosome integrity were studied. The experimental results showed that the STJ+ dilution medium in which the biologically active preparation extracted from the cyanobacteria was introduced gave the possibility to obtain after thawing the mobility of 49.2+1.7, spermatozoa with rectilinear movements VAP – 105.5+2 ,7, VSL-91.5+4.5, VCL-170.7+8.5, sperm abnormalities -14.5%, acrosome integrity 24.4+0.4 compared to the other GTS and STJ dilution media, which gave us the possibility to recommend for the cryopreservation of ram sperm the STJ+ medium in which the biologically active preparation was introduced as an additional component in a concentration of 0.4-0.7% for the cryopreservation of ram sperm.