Specificity of membrane-bound enzymes activity over the cryopreservation of farm animals sperm

Abstract

The intensity of the vital processes in spermatozoa in addition to the transmembrane defects is determined through activity of membrane-bound enzymes. The purpose of our research was to study the activity of membrane-dependent enzymes in the sperm of different animals species at cryopreservation. In experimental studies were used physiological, cryobiological and statistical methods that were held on the plasmatic membranes of sperm of the boar of Large White breed and the roosters of Rhode Island Red breed. The obtained results in the study of the activity of Mg+2 (Na+ + K+ ) - ATPase in the membranes of spermatozoa of the diluted sperm, and after freezing and thawing of sperm of rooster and boar attest substantial destruction of the plasmatic membranes of these breeds. Activity of 5’-nucleotidase in isolated rich fractions of plasmatic membranes of native spermatozoa of rooster and boar not suffer essential changes at cryopreservation of sperm these species. Our research revealed minor changes in the activity of alkaline phosphatase in the membranes of native and frozen-thawed rooster and boar spermatozoa. The study of the above enzymes of plasmatic membranes of spermatozoa of rooster and boar are certain theoretical and practical interest. They allow obtaining new data on the chemical composition of the membranes, reveal the specific relationship between the content and ratio of the structural components of membranes and the resistance of rooster and boar spermatozoa to low temperatures.