Cryogenic changes of lipid during preservation of sperm animal farm

Abstract

The solution of the problems of cryopreservation of sperm of farm animals is determined largely by the intermolecular interactions of cellular components of reproductive cells. Therefore, the aim of the research was the study of the contribution of the lipid components in the manifestation of adaptive-compensatory changes of gametes of animals in hypothermic conditions. Experimental investigations were carried out using sperm of the boars of Large White breed, the roosters of Rhode Island Red breed and the bulls of Black and White breed. Isolation of plasmatic membranes of spermatozoa was conducted according to the method developed by Ivanov and Porfiry (1981) in our modification (ɇɚɭɤ et. al, 1993). The obtained results proved that the phospholipids in the process of cryopreservation of sperm of farm animals are exposed to the greatest changes, while the cholesterol content is more stable. Also, the molar ratio phospholipids:cholesterol was changed in the direction of value “1” after cooling and freezing of sperm. Decrease of the ratio phospholipids:cholesterol is one of the mechanisms in the system of adaptive-compensatory reactions of spermatozoa at the influence of low temperatures. The study of the adaptation mechanisms at the cellular and molecular levels attracted the attention of an increasing number of researchers, because in this direction is possible prospects of solving the problems of ɫryogenic changes during preservation of sperm.