https://repository.utm.md/handle/5014/8302 2026-06-30T11:38:34Z 2026-06-30T11:38:34Z CIBOTARU, Elena DARIE, Grigore DJENJERA, Irina CHISALITA, Oleg https://repository.utm.md/handle/5014/36672 2026-06-29T16:06:45Z 2026-01-01T00:00:00Z

Research on the influence of dilution media on ram sperm cryopreservation CIBOTARU, Elena; DARIE, Grigore; DJENJERA, Irina; CHISALITA, Oleg In the study, ram sperm from the Moldovan Karakul breed bred in the experimental section of the Scientific Institute of Biotechnology Practices in Animal Husbandry and Veterinary Medicine. Ejaculates with a mobility of no less than 70% and a concentration of 2.0 billion/ml were admitted for processing. For dilution, GTJ, STJ and STJ+ dilution media were used, in which the biologically active preparation extracted from cyanobacteria at the Institute of Microbiology and Biotechnology of the Technical University of Moldova was introduced as an additional component. After resuscitation, the mobility, rectilinear movements of the spermatozoa, acrosome integrity were studied. The experimental results showed that the STJ+ dilution medium in which the biologically active preparation extracted from the cyanobacteria was introduced gave the possibility to obtain after thawing the mobility of 49.2+1.7, spermatozoa with rectilinear movements VAP – 105.5+2 ,7, VSL-91.5+4.5, VCL-170.7+8.5, sperm abnormalities -14.5%, acrosome integrity 24.4+0.4 compared to the other GTS and STJ dilution media, which gave us the possibility to recommend for the cryopreservation of ram sperm the STJ+ medium in which the biologically active preparation was introduced as an additional component in a concentration of 0.4-0.7% for the cryopreservation of ram sperm.

2026-01-01T00:00:00Z CIBOTARU, Elena DARIE, Grigore DJENJERA, Irina ROTARI, S. https://repository.utm.md/handle/5014/36671 2026-06-29T14:16:28Z 2025-01-01T00:00:00Z

The role of the biologically active preparation in optimizing spermatogenesis in breeding boars CIBOTARU, Elena; DARIE, Grigore; DJENJERA, Irina; ROTARI, S. The study aimed to evaluate the role of a complex biologically active preparation in optimizing spermatogenesis in breeding boars. The results showed an increase in ejaculate volume in the experimental group (202.5 ± 29.5 ml compared to 168.0 ± 8.5 ml in the control group), as well as an improvement in the qualitative parameters of the semen. Specifically, the percentage of motile spermatozoa increased to 92.4 ± 0.4% compared to 92.0 ± 0.4% in the control group. Additionally, a significant increase in the percentage of progressively motile spermatozoa was observed (72.3 ± 2.8% in the experimental group versus 65.3 ± 2.9% in the control group). Furthermore, the proportion of morphologically normal spermatozoa was higher in the experimental group (78.3 ± 2.9%) than in the control group (69.8 ± 3.4%). Thus, the administration of the complex biologically active preparation optimized the spermatogenesis process in breeding boars by increasing ejaculate volume and improving qualitative parameters (motility, progressive motility, and normal morphology), indicating its potential benefit in supporting reproductive capacity.

2025-01-01T00:00:00Z CIBOTARU, Elena BRADU, Nina ROTARI, Doina DARIE, Grigore DJENJERA, Irina https://repository.utm.md/handle/5014/36669 2026-06-26T16:56:58Z 2022-01-01T00:00:00Z

Research on ram sperm freezing CIBOTARU, Elena; BRADU, Nina; ROTARI, Doina; DARIE, Grigore; DJENJERA, Irina The aim of the research was to perfect the ram sperm freezing protocols and develop the dilution medium for cryopreservation. The research was carried out on ram sperm, collected with the help of the artificial vagina. Ejaculates with a mobility of no less than 70% and a concentration of spermatozoa in the ejaculate of 2.5 billion/ml were taken for processing. Dilution medium consisting of sucrose, sodium citrate and egg yolk was used to dilute the semen. As an additional component in the composition of the environment, the preparation MP was introduced in a concentration from 0.1 to 1.0%/v. Post-thaw motility, spermatozoa with rectilinear movements, spermatozoa abnormalities, and spermatozoa with damaged acrosome were assessed. The results of the research demonstrated that the introduction in the dilution medium of the MP preparation in a concentration of 0.5-0.7% as an additional component allowed the mobility to be maintained at the level of 57% and the number of spermatozoa with rectilinear movements of 26-27 % after thawing, or 3.8% higher compared to the control group after thawing, it also allowed to decrease the percentage of spermatozoa with abnormalities by 2.9% and spermatozoa with damaged acrosome by 2%.

2022-01-01T00:00:00Z ROTARI, Doina CIBOTARU, Elena DARIE, Grigore BRADU, Nina DJENJERA, Irina https://repository.utm.md/handle/5014/36668 2026-06-26T15:47:33Z 2023-01-01T00:00:00Z

Spermogram in rams in the secondary session of reproduction under the influence of biologically active drugs ROTARI, Doina; CIBOTARU, Elena; DARIE, Grigore; BRADU, Nina; DJENJERA, Irina The influence of the bioactive drugs ZooBioR-1. and ZooBioR-2, on the stimulation of spermatogenesis in breeding rams in the secondary breeding season was studied. It was demonstrated that the drugs ZooBioR-1 and ZooBioR-2 had a positive influence on the quality of ejaculates taken from breeding rams in the secondary breeding season; the volume of the ejaculate at the end of the experiment was 0.8±0.04 ml (P≤0.001), the mobility of the spermatozoa increased up to 80.0±1.6%, the concentration of spermatozoa in the ejaculate increased up to 2.34±0 .09 billion/ml, the concentration of testosterone in the blood increased up to 6.0±0.1ng/ml, compared to the control group, indices that correspond to the requirements for ejaculates admitted for processing.

2023-01-01T00:00:00Z