https://repository.utm.md/handle/5014/8302 2026-06-24T22:22:48Z https://repository.utm.md/handle/5014/36566 The Influence of Stimulative Feeding of Nurse Bees on Royal Jelly Production EREMIA, Nicolae; JEREGHI, Vitalie The aim of the research was to evaluate the influence of stimulative feeding of nurse bees on increasing larval acceptance for rearing, queen cell dimensions (diameter, length, and the mass of the queen cell containing royal jelly and the larva), as well as the amount of royal jelly obtained from a single queen cell, per rearing cycle and in total over the course of three cycles. In the first cycle, each experimental colony received one frame containing 45 grafted larvae. In the second cycle, two frames containing 90 grafted larvae were provided, while in the third cycle, three frames containing 135 grafted larvae were introduced. The nurse bees were fed daily with one liter of a mixture consisting of sugar syrup and the biostimulator MF-SIB-49, beginning from the moment the frames with grafted larvae were introduced and continuing for three consecutive days, until the day on which the frames were removed for royal jelly harvesting. This procedure was applied during the second and third cycles as well. Based on the evaluation performed, it was found that, in the absence of a maintenance nectar flow, feeding nurse bees with a mixture of sugar syrup and biostimulator stimulates royal jelly secretion required for the rearing of grafted larvae. This leads to an increase in the number of grafted larvae accepted for rearing, improves the developmental parameters of queen cells (length, diameter, and the mass of the queen cell together with royal jelly and larva), and increases the amount of royal jelly obtained from a single queen cell, both per cycle and in total across three cycles. The total amount of royal jelly obtained from one bee colony over the three cycles was 66.0 g, which was 32.0–45.4% higher than in the control group. 2026-01-01T00:00:00Z https://repository.utm.md/handle/5014/36558 Study on the Use of Different Types of Artificial Honeycombs in Bee Exploitation MARDARI, Tatiana The purpose of the research is to study the use and evaluation of the growth of different types of artificial honeycombs. Following the assessment of the intensity of acceptance and cell formation by bees of different types of artificial honeycombs: coloured, simple, ecological and traditional ones, it was revealed that newly built honeycombs have a light-yellow colour and worker bees give priority to the construction of cells from wax capping and to the simple ones compared to the traditional ones. Worker bees gave priority to the coloured artificial honeycomb, and it was accepted for cell growth, keeping the red colour compared to the simple and traditional honeycombs. Artificial combs made of wax capping (ecological) are the most accepted for cell formation by bees and honeycomb construction. 2026-01-01T00:00:00Z https://repository.utm.md/handle/5014/36517 Research on cryopreservation of ram semen DARIE, Grigorie; CIBOTARU, Elena; BRADU, Nina; DJENJERA, Irina The research was carried out on semen obtained from Karakul and Tigaie rams. There was reviled a high individual variability of sexual behaviour in Bethaimer and Sulfoc ejaculate efficacy in the cryopreservation process, the dilution medium for cryopreservation of the seminal material was proposed. The evaluation of the results of ram semen freezing supposed the evaluation of the ejaculates obtained from rams of various breeds, the cryopreservation, the storage of the semen and its use for the sowing of the sheep and the stock of semen material in the gene banks. 2017-01-01T00:00:00Z https://repository.utm.md/handle/5014/36516 Comparative analysis of cryopreserved ram sperm parameters depending on the dilution medium DARIE, Grigore; CIBOTARU, Elena; DJENJERA, Irina; BRADU, Nina To improve the effectiveness of long-term sperm storage outside the animal’s body, it is necessary to enhance the protective media that help reduce the impact of cryodamaging factors on sperm. In this study, sperm from Assaf breed rams was used. Sperm was collected from the rams twice a week using an artificial vagina, which ensured high-quality material. For analysis, ejaculates with at least 70% motility and a concentration of 2,0 to 2,5 billion sperm per milliliter were selected. The aim of the study was to compare the parameters of cryopreserved sperm depending on the synthetic media used: GTJ, STJ+MP, and STJ+IMB-2. This allowed for the determination of the most suitable extender to ensure a higher fertilization rate. During the study, the main parameters of cryopreserved sperm were analyzed, including motility, percentage of pathological sperm forms, plasma membrane integrity (based on the hypoosmotic swelling test results), and acrosome integrity. The highest values were achieved with samples diluted with the STJ+IMB-2 medium. In this group, the following results were obtained: motility – 54,0±0,4%, velocity (VAP) – 91,8±3,4%, VSL – 75,5±3,9%, VCL – 132,0±3,8%, percentage of anomalies – 16,75%, acrosome integrity – 24,15±0,31%, and plasma membrane integrity was 65,6±0.7%. The obtained data suggest that the STJ+IMB-2 medium provided the most effective protection for sperm during cryopreservation compared to other media such as GTJ and STJ+MP. These results highlight the importance of selecting an appropriate medium to optimize cryopreservation processes and improve fertilization rates. 2025-01-01T00:00:00Z